Cytogenetics Mcqs

Q:

Which of the following drugs effect caspases?

A) Oblimerson B) Pikan083
C) Tenovin D) Apoptin
 
Answer & Explanation Answer: D) Apoptin

Explanation: Apoptin, a caspase-inducing agent, has been reported to induce apoptosis in malignant but not in normal cells. Small molecules caspase activators are peptides which contain the arginine-glycine-aspartate motif. They induce activation of procaspase 3.

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16
Q:

Which fluorescent dye can be used for red fluorescence?

A) Rhodamine B) Fluorescein
C) Carmine D) DAPI
 
Answer & Explanation Answer: A) Rhodamine

Explanation: The Rhodamine gives red fluorescence while the fluorescein gives a green fluorescence. Carmine is a red dye that is used in food but it’s not used for immunological methods. DAPI stains the nucleus blue.

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23
Q:

How are the cells sorted?

A) By dilution plating until there are only single cell in each well of microtitre plate B) By the differential weight
C) By electrostatic force D) By magnetic force
 
Answer & Explanation Answer: C) By electrostatic force

Explanation: As the cells exit the nozzle of the flow cytometer a small amount of charge is added to the droplet. Then it passes between the two places which induce the droplet to be properly separated depending on its label.

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19
Q:

In a flow cytometer the particles are analyzed all at a time.

A) True B) False
 
Answer & Explanation Answer: B) False

Explanation: It is ensured by dilution that in a flow cytometer the particles pass in a single file. Then they are individually identified and sorted.

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15
Q:

In flow cytometry which of the combination is impossible?

A) Both the labeled antigens in same particle B) None of the labeled antigens on a particle
C) One of the labeled antigen D) All can be true
 
Answer & Explanation Answer: D) All can be true

Explanation: In flow cytometry we can have all the above combinations. The cells can even be sorted according to the antigens present on them.

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15
Q:

Flow cytometry uses_____________

A) Heavy isotope B) Radioactive elements
C) Immunological techniques D) Energy content
 
Answer & Explanation Answer: C) Immunological techniques

Explanation: The process of flow cytometry is based on immune fluorescence where a laser is used to irradiate the fluorescent particle bound to a particle that determines its fete.

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27
Q:

Pikan083 can act on p53 mutants and induce cancer.

A) True B) False
 
Answer & Explanation Answer: A) True

Explanation: Mutant p53 doesn’t bind to the promoter and hence can’t induce apoptosis by transcribing. Pikan083 can bind to and activate mutant p53 in proliferative cells leading to their apoptosis.

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13
Q:

Tenovins are__________________________

A) Bcl2 sequestering B) Cytochrome releasing
C) Mutant p53 repairing D) p53 activating
 
Answer & Explanation Answer: D) p53 activating

Explanation: When tenovin is administered, this small molecule activates p53 which in turn leads to apoptosis. This is used as a drug to treat cancer.

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