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Genetic Engineering Mcqs

Q:

Which of the following is not suitable if the PCR product is non-specific?

A) Adjusting the concentration of magnesium ions B) Increasing annealing temperature
C) Using touchdown PCR D) Using inverse PCR
 
Answer & Explanation Answer: D) Using inverse PCR

Explanation: In case, products are non-specific then sequence can’t be known directly. Optimization of PCR should be carried out and various other strategies should be used such as adjusting the concentration of magnesium ions, increasing annealing temperature or using touch-down PCR.

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39
Q:

Amplification of specific region can be done by using primers for specific regions. If the PCR product is ______ and is in sufficient quantity, then sequence can be determined ________

A) non-specific, directly B) non-specific, indirectly or directly
C) specific, directly D) specific, indirectly
 
Answer & Explanation Answer: C) specific, directly

Explanation: Amplification of specific region can be done by using primers for specific regions. If the PCR product is specific, it means that only a single band is obtained in gel electrophoresis and is insufficient quantity then the sequence can be determined directly.

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94
Q:

For carrying out gene manipulation, the use of cultured cells is _____ transgenic organisms.

A) less reliable B) more reliable
C) may be less or more reliable D) is same reliable as
 
Answer & Explanation Answer: A) less reliable

Explanation: For carrying out gene manipulation, the use of cultured cells is less reliable than that of transgenic organisms. Thus, these transgenic organisms are used greatly.

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39
Q:

If controlled inactivation of a gene is carried out and some of the consequences when inactivation of a target gene is deleterious are avoided. It is referred as ____________

A) specialized gene targeting B) controlled gene targeting
C) conditional gene targeting D) specific gene targeting
 
Answer & Explanation Answer: C) conditional gene targeting

Explanation: Conditional gene targeting is that where controlled inactivation is carried out. If inactivation leads to deleterious effects then they are avoided.

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43
Q:

The gene targeting approach produces individuals which are ______ for inactivation of the gene.

A) homozygous B) heterozygous
C) either only homozygous or only heterozygous D) both heterozygous and homozygous
 
Answer & Explanation Answer: B) heterozygous

Explanation: The gene targeting approach produces individuals which are heterozygous for inactivation of the gene. But it is necessary to generate homozygotes and they are produced by crossing heterozygous individuals and then screening is carried out.

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64
Q:

It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting.

A) True B) False
 
Answer & Explanation Answer: A) True

Explanation: It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct and it is carried out by southern blotting. DNA from cultures derived from individual transformed cells is used for screening.

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39
Q:

Integration events may be insertional involving _____ crossover or replacement involving _____ crossovers.

A) single, single B) double, double
C) double, single D) single, double
 
Answer & Explanation Answer: D) single, double

Explanation: Integration can be done by either insertion or replacement. Insertion is carried out by using single crossover and replacement is carried out by double crossover.

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53
Q:

If a gene is inactivated by gene targeting then it is called as ____________

A) knock-in gene B) knock-out gene
C) gene disruption D) insertional inactivation
 
Answer & Explanation Answer: B) knock-out gene

Explanation: If a gene is inactivated by gene targeting then it is called a knock-out gene. If a gene is replaced by some other gene then it is called as knocking in.

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