Protein Engineering Mcqs

Q:

Both the subunits of heterodimers are involved in tRNA binding.

A) False B) True
 
Answer & Explanation Answer: B) True

Explanation: The above statement is true. Both the subunits of heterodimers are involved in tRNA binding. The heterodimer is a fully refolded, active molecule, in which one subunit can complement a lesion on the other subunit.

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13
Q:

The tRNA makes contact with the synthetase at only a single specific position.

A) True B) False
 
Answer & Explanation Answer: B) False

Explanation: The above statement is false. The tRNA makes contact with the synthetase over a wide region, and in particular at both the acceptor arm and anticodon stem and loop. Hence, the tRNA does not make contact with the synthetase at only a single specific position.

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18
Q:

Tyrosine is activated at an amino group with ATP to form tyrosyl adenylate.

A) True B) False
 
Answer & Explanation Answer: B) False

Explanation: Not available for this question

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16
Q:

Which of the following amino acids can be used in place of lysine, arginine, and histidine to remove the electrostatic charge but retain the hydrophilic nature of the side chain?

A) Aspartic acid and glutamic acid B) Aspartic acid and glutamine
C) Asparagine and glutamic acid D) Asparagine and glutamine
 
Answer & Explanation Answer: D) Asparagine and glutamine

Explanation: Asparagine and glutamine can be used in place of lysine, arginine, and histidine to remove the electrostatic charge but retain the hydrophilic nature of the side chain. Aspartic acid and glutamic acid cannot be used in place of lysine, arginine, and histidine to remove the electrostatic charge and retain the hydrophilic nature of the side chain. Asparagine and glutamine contain neutral but polar side chains.

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23
Q:

The C-terminal domain of tyrosyl-tRNA synthetase contains major binding determinants for the tRNA.

A) False B) True
 
Answer & Explanation Answer: B) True

Explanation: The above statement is true. The C-terminal domain of tyrosyl-tRNA synthetase contains major binding determinants for the tRNA. The C-terminal domain was excised at the level of the cloned gene and the kinetics of the N-terminal domain was assayed. The kinetics of the tyrosine activation were identical for the wild type and the truncated enzyme, but the truncated enzyme did not charge or bind tRNA.

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18
Q:

Which of the following enzyme catalyzes the charging of tRNATyr with tyrosine?

A) Tyrosine synthetase B) RNA activating enzyme
C) Tyrosyl-tRNA synthase D) Tyrosyl-tRNA synthetase
 
Answer & Explanation Answer: D) Tyrosyl-tRNA synthetase

Explanation: Tyrosyl-tRNA synthetase catalyzes the charging of tRNATyr with tyrosine. It is a two-stage reaction in which the tyrosine is first activated at its carboxyl group with ATP to form tyrosyl adenylate. Tyrosine synthetase, RNA activating enzyme, or Tyrosyl-tRNA synthase do not catalyze the charging of tRNATyr with tyrosine.

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14
Q:

Protein-based subunit vaccines initiate T cell-dependent activation of B cells.

A) False B) True
 
Answer & Explanation Answer: B) True

Explanation: The above statement is true. Protein-based subunit vaccines initiate T cell-dependent activation of B cells, a process characterized by a more robust immune response, affinity maturation, and immunological memory. On the contrary, polysaccharide vaccines are unable to recruit the assistance of T helper cells and thus rely on T cell-dependent activation alone.

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11
Q:

Most viral vaccines are thought to work by which of the following technique?

A) Inducing the production of antigens B) Inducing the production of cell wall
C) Inducing the production of cytosolic proteins D) Inducing the production of antibodies
 
Answer & Explanation Answer: D) Inducing the production of antibodies

Explanation: Most viral vaccines are thought to work by inducing the production of antibodies, that block infection or reduce viral load, thereby providing host protection or blunting infection such that cellular immunity can be effective. Inducing the production of antigens, inducing the production of a cell wall, and inducing the production of cytosolic proteins are not the techniques by which most of the viral vaccines work.

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