Vector Biology & Gene Manipulation Mcqs


Incorporation of ribozymes into antisense RNA leads to their __________

A) Activity B) Temperature change
C) Cleavage D) Regeneration
Answer & Explanation Answer: C) Cleavage

Explanation: The incorporation of ribozyme catalytic centers into antisense RNA allows the ribozyme to be particularly targeted and then cleaved and degraded.

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Southern blot hybridization is done for testing the presence of _________ in the selection of transformants.

A) Non-selected DNA B) Selected plasmid DNA
C) Genomic DNA D) Selected genomic DNA
Answer & Explanation Answer: A) Non-selected DNA

Explanation: To obtain co-transformants, cells were transfected with HSV Tk gene and well-defined plasmid DNA. Cells selected on HAT medium are then tested by southern blotting for the presence of non-selected DNA.

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In the salvage pathway, nucleotides are synthesized by the recycling of __________

C) DNA, RNA D) Sugars
Answer & Explanation Answer: C) DNA, RNA

Explanation: In mammals, nucleotides are produced via two alternate routes, the de novo and the salvage pathway. Salvage pathway recycles nucleotides from DNA and RNA.

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Ribozymes are _________ molecules.

A) Toxic B) Large
C) DNA D) Catalytic
Answer & Explanation Answer: D) Catalytic

Explanation: Ribozymes are catalytic molecules that destroy targeted MRNAs. They carry out site-specific cleavage and ligation reactions.

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Insertional mutagenesis facilitates __________

A) Cloning B) PCR
C) Recombination D) Centrifugation
Answer & Explanation Answer: C) Recombination

Explanation: Insertional mutagenesis leaves a DNA tag in the interrupted gene, which facilitates cloning and gene identification. It is the most popular mutagenesis strategy.

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Genome-wide random mutagenesis is applicable to _________

A) Fish B) Mammals
C) All organisms D) Bacteria
Answer & Explanation Answer: C) All organisms

Explanation: Saturation mutagenesis has been used for many years to identify mutations affecting specific crucial biological processes.

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The EUROFAN project involves the use of _________ generated cassettes.

A) PCR B) Ultracentrifugation
C) Electroporation D) Cloning
Answer & Explanation Answer: A) PCR

Explanation: The EUROFAN project involves the use of PCR-generated cassettes in which a selectable marker is placed between 50 basepair elements.

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The only organism in which systematic gene targeting has been achieved is ____________

A) Mouse B) Drosophila
C) Fish D) Yeast
Answer & Explanation Answer: D) Yeast

Explanation: The only organism in which systematic gene targeting has been achieved is the yeast Saccharomyces Cerevisiae. Its genome contains about 6000 open reading frames.

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